Orotic acid-treated hepatocyte carcinoma cells resist steatosis by modification of fatty acid metabolism

Johanna Matilainen, Kirsi Rilla, Anne-Mari Mustonen, Reijo Kakela, Petteri Nieminen: Institute of Biomedicine, University of Eastern Finland; Helsinki University Lipidomics Unit, University of Helsinki Finland .

INTRODUCTION: Orotic acid (OA) has been intensively utilized to induce fatty liver in rats. However, the capacity of OA to cause liver steatosis is species-specific, which may be due to the regulation of transcription factors mediating hepatic lipogenic events. Despite this, previous research evaluating OA-mediated hepatocytic lipidosis in cell types other than primary rat hepatocytes remains sparse, and there are no previous data about the detailed fatty acid (FA) composition of the OA-treated cells nor about how the available FA are processed.

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